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Addgene inc alk4 wt
Alk4 Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alk4+wt/pcDNA3-ALK4+wt+(Plasmid+%2380879)/pm41408046-231-1-25
Average 93 stars, based on 2 article reviews
alk4 wt - by Bioz Stars, 2026-09
93/100 stars

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Related Articles

Stable Transfection:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Expressing:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Control:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

shRNA:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Injection:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Imaging:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Staining:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Two Tailed Test:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

MANN-WHITNEY:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

CRISPR:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Clone Assay:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Western Blot:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Soft Agar Assay:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Migration:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Transwell Migration Assay:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Live Cell Imaging:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Gene Expression:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Microscopy:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Dominant Negative Mutation:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Quantitative RT-PCR:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Binding Assay:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Transfection:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Blocking Assay:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Incubation:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

In Vitro:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Knock-Out:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Functional Assay:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Transformation Assay:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Generated:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Modification:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Labeling:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Magnetic Beads:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

In Vivo:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).

Comparison:

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation.
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid [77] was provided by Dr. Anne Klibanski.

Article Title: Loss of ALK4 promotes cancer progression through regulating TGF-β receptor N-glycosylation
Article Snippet: The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.The human pancreatic cell lines PANC-1 and HPNE (ATCC) were maintained in DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879), ALK4 CA (Cat# 27223), TβRII DN (Cat# 12640), TβRI (Cat# 14831), and TβRII (Cat# 11766) DNA plasmids were purchased from Addgene.. The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.The ALK4 DN DNA plasmid was provided by Dr. Anne Klibanski.

Article Title: A Model for the Diversity Explosion Fundamental to Metastasis
Article Snippet: Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.Panc1 human pancreatic cell lines were obtained from ATCC and each was cultured in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin.. The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.. The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).The CRISPR ALK4 knockout cells were generated using pLentiCRISPRv2-puro vector (Addgene, Cat# 98290).



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